Colorimetric determination of serum guanase activity.
نویسنده
چکیده
A colorimetric method for the determination of serum guanase has been developed whereby ammonia, formed by the deamination of guanine, is measured by the phenatehypochlorite reaction. Variations in substrate concentration, time of incubation, pH, temperature, and concentration of enzyme have been investigated with respect to enzyme activity. The determination of serum guanase appears to be useful in the differential diagnosis of jaundice.
منابع مشابه
Simple, rapid determination of serum guanase activity with the Hitachi 736 automated discrete analyzer.
In this new method for determining serum guanase activity by use of the Hitachi 736-40 automated analyzer, serum is incubated with a mixture of xanthine oxidase, superoxide dismutase, and catalase; a reagent containing KCN, guanine, nitrotetrazolium blue, and Triton X-100 is added; and the increase in absorbance at 570 and 660 nm is measured for 2.4 min. Only 20 microL of sample is required, an...
متن کاملKinetic measurement of guanine deaminase in serum with a centrifugal analyzer.
We describe an enzymic, one-step kinetic method for determination of guanine deaminase (guanase, EC 3.5.4.3) in serum with a centrifugal analyzer. A combined enzyme-substrate system consists of the enzymes xanthine oxidase, catalase, and aldehyde dehydrogenase, the coenzyme NAD+, the substrate guanine, and ethanol in tris(hydroxymethyl)methylamine buffer, with KCl added as activator for aldehyd...
متن کاملA simple colorimetric method for the determination of serum alpha-hydroxybutyric dehydrogenase activity.
An accurate, simple colorimetric method for the determination of serum alpha-hydroxybutyric dehydrogenase activity is described. The method is suitable for routine laboratory use.
متن کاملA simple and sensitive radiochemical assay for plasma guanase.
A simple and sensitive radiochemical micro-assay has been developed for the determination of plasma guanase activity. The method is based upon the measurement of the conversion of 14C-labeled guanine to xanthine, catalysed by the enzyme guanase (guanine aminohydrolase;EC 3.5.4.3). Using this method, the catalytic activity in the plasma of adult healthy controls was 0.040 +/- 0.09 nmol/h.mg prot...
متن کاملA fluorometric determination of trypsin-like amidase activity and activity of trypsin inhibitors in serum.
The activity of crystalline trypsin and the trypsin-like amidase activity and the activity of trypsin inhibitors in serum were measured fluorometically, with a-benzoyl--L-arginine--na phthylamide (BANA) as substrate. The method is simpler and more sensitive than the colorimetric method based on the Bratton-Marshall reaction. Serum of pancreatectomized dogs hydrolyzed BANA at a significant rate,...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Clinical chemistry
دوره 12 4 شماره
صفحات -
تاریخ انتشار 1966